Journal: Brain and Behavior
Article Title: Discovery of restless legs syndrome plasmatic biomarkers by proteomic analysis
doi: 10.1002/brb3.1062
Figure Lengend Snippet: Representative 2D gel maps. (a) Control group, (b) MMS‐RLS patients group, (c) HS‐RLS patients group. In gel images are marked the protein spots significantly different among the three groups, identified by ESI‐Q‐ToF‐MS. Panel (a) shows the decreased protein spots, panel (b) illustrates the spots increased in both RLS groups vs. controls, and panel (c) the spots increased in HS‐RLS group vs. MMS‐RLS and control groups. Alphanumeric codes in the figures correspond to those listed in Table . Each gel map reports the molecular weight (MW) standard protein ladder, expressed in kilodalton (kDa) (All Blue Precision Plus Protein ™ Standards, Bio‐Rad). First dimension separation: IPG strip, pH 3–10. Second‐dimension separation: 10% polyacrylamide gels
Article Snippet: The gel images were acquired by a calibrated densitometer (GS‐800, Bio‐Rad) and analyzed by the PDQuest 2‐D image analysis software (Bio‐Rad), as previously reported (Bellei et al., ).
Techniques: Two-Dimensional Gel Electrophoresis, Control, Molecular Weight, Stripping Membranes